Culture, characterization and differentiation of cells from buffalo (Bubalus bubalis) amnion

Isolation and characterization of luteal cells in buffalo (Bubalus bubalis).

The objective of the study was to evaluate the morphological and functional characteristics of luteal cells isolated from buffalo ovary. Luteal cells exhibited columnar morphology and contact inhibition at the stage of confluence. Protein concentrations increased linearly from Day 3 to Day 7 of culture. DNA concentrations increased from Day 3 to Day 5 and then declined to Day 7 of culture. PGF2...

Isolation and characterization of buffalo (bubalus bubalis) amniotic

Optimization of Buffalo (Bubalus bubalis) Embryonic Stem Cell Culture System

OBJECTIVE In order to retain an undifferentiated pluripotent state, embryonic stem (ES) cells have to be cultured on feeder cell layers. However, use of feeder layers limits stem cell research, since experimental data may result from a combined ES cell and feeder cell response to various stimuli. MATERIALS AND METHODS In this experimental study, a buffalo ES cell line was established from in ...

Characterization of PRLR and PPARGC1A genes in buffalo (Bubalus bubalis)

More than 40 million households in India depend at least partially on livestock production. Buffaloes are one of the major milk producers in India. The prolactin receptor (PRLR) gene and peroxisome proliferators activated receptor-γ coactivator 1-alpha (PPARGC1A) gene are reportedly associated with milk protein and milk fat yields in Bos taurus. In this study, we sequenced the PRLR and PPARGC1A...

Biochemical characterization of buffalo (Bubalus bubalis) milk lysozyme.

Lysozyme, a low-molecular weight basic protein, is an important component of the antibacterial system in milk. Lysozyme activity is higher in buffalo milk (60±3.9r 10 units/ml) than in bovine milk (29.1±1.5r10 units/ ml). Buffalo colostrum contains five-times more lysozyme activity than mature milk (Priyadarshini & Kansal, 2002a). Lysozyme activity in buffalo milk is not influenced by the parit...